We have an LCQ Deca XP (Thermo-Finnigan) Ion Trap mass detector linked to a Surveyor chromatographic system and an Agilent 1100 HPLC system with Diode Array detection. We have over 20 years experience in the analysis of nucleotides and their metabolites in the heart, other tissues and cell extracts. We have established several new procedures for the chromatographic analysis of nucleotides and their metabolites and have published >100 papers using these techniques. Our latest achievements include the development of an anion exchange chromatographic method for nucleotide analysis with mass detection and high sensitivity methods for the study of metabolic fluxes in the Krebs cycle and of glycolysis in vivo by metabolite isotopomer analysis.

We are interested in the development of collaboration and custom analysis using these methods. In particular, we can provide on site research training for PhD students combined with analysis of samples for their thesis. This includes general training in HPLC with UV or MS detection and training in a specific established method including:

1) Nucleotides, their catabolites and other high energy phosphates analysis in tissue and cell extracts by HPLC with Diode Array Detection. 2) Nucleotides and adenosine analysis in biological samples using HPLC with mass detection. 3) Amino acid analysis in plasma or protein hydrolysates using HPLC/MS. 4) Glucose, lactate, alanine and glutamate isotopomer analysis by HPLC/MS. 5) Highly sensitive hydroxyproline concentration analysis in biological samples by HPLC/MS. 6) Measurement of plasma clenbuterol concentration using HPLC/MS. 7) Analysis of nicotinamide and its metabolites: 2PY, 4PY, M2PY, M4PY, N-methyl-nicotinamide in biological samples by HPLC/MS.

Our research involving HPLC/MS

Please contact Dr RT Smolenski for further information.

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